protein array การใช้

• A drawback of DNA and protein arrays is that they are neither reconfigurable nor scalable after manufacture.
• This presents a challenge in maintaining protein arrays in a stable condition over extended periods of time.
• Despite the success of first chip, it was a greater challenge for protein arrays to be implemented.
• Several technology platforms, including reverse phase protein arrays and advanced microscopy, support the research activities with cutting-edge technology.
• They differ from analytical arrays in that functional protein arrays are composed of arrays containing full-length functional proteins or protein domains.
• Common sources of cell extracts used in cell-free protein array technology include wheat germ, " Escherichia coli ", and rabbit reticulocyte.
• In the most common type of protein array, robots place large numbers of proteins or their ligands onto a coated solid support in a pre-defined pattern.
• A protease specific protein array based on immobilized antibodies designed to capture specific proteases from biological samples offers a step up in analysis of protein levels beyond transcript expression.
• Cell-free protein array technology attempts to simplify protein microarray construction by bypassing the need to express the proteins in bacteria cells and the subsequent need to purify them.
• Examples of in situ techniques are PISA ( protein in situ array ), NAPPA ( nucleic acid programmable protein array ) and DAPA ( DNA array to protein array ).
• Examples of in situ techniques are PISA ( protein in situ array ), NAPPA ( nucleic acid programmable protein array ) and DAPA ( DNA array to protein array ).
• One example : Corning is developing a protein array, made in a manner similar to the DNA Microarray, that can be used to check the potential of various substances for use as drugs.
• Studies in the past two decades have identified the genetic determinants of longevity, using approaches such as genome-wide RNA interference ( RNAi ) and mutagenesis screens, microarrays, and protein arrays.
• DNA array to protein array ( DAPA ) is a method developed in 2007 to repeatedly produce protein arrays by  printing them from a single DNA template array, on demand ( Figure 5 ).
• DNA array to protein array ( DAPA ) is a method developed in 2007 to repeatedly produce protein arrays by  printing them from a single DNA template array, on demand ( Figure 5 ).
• The traditional methods of producing protein arrays require the separate " in vivo " expression of hundreds or thousands of proteins, followed by separate purification and immobilization of the proteins on a solid surface.
• In addition to trio-based whole-genome sequencing for each individual, ITMI analyzes epigenetic ( currently through DNA methylation studies ), RNA expression ( RNAseq ), and proteomic ( e . g ., with mass spectrophotometry and reverse phase protein arrays ) data.
• Similar to a DNA microarray, a protein array is a miniature array where a multitude of different capture agents, most frequently monoclonal antibodies, are deposited on a chip surface; they are used to determine the presence and / or amount of proteins in biological samples, e . g ., blood.
• Functional protein microarrays ( also known as target protein arrays ) are constructed by immobilising large numbers of purified proteins and are used to identify protein protein, protein DNA, protein RNA, protein phospholipid, and protein small-molecule interactions, to assay enzymatic activity and to detect antibodies and demonstrate their specificity.